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Image Search Results
Journal: EBioMedicine
Article Title: Integrated analysis of DNA methylome and transcriptome identified CREB5 as a novel risk gene contributing to recurrent pregnancy loss
doi: 10.1016/j.ebiom.2018.07.042
Figure Lengend Snippet: DNA methylation patterns in RPL and controls. (a) Circular representation of whole genome DNA methylation level for RPL and controls. CpG methylation level were averaged per Mb in the genome and represent as histograms and heatmaps. (b) Distributions of hyper-methylated and hypo-methylated DMPs were investigated throughout each genomic region, FDR < 0·05. (c) Genomic locations divided into island, opensea, shelf and shore according to the distance between CpG island and DMPs, FDR < 0·05. (d) Manhattan plot showing P value based on DMPs analyses. The two horizontal lines is the suggestive DNA methylation chip significance threshold cutoff. (e) A volcano plot of the distribution between FDR (adjusted P value) and difference in β value (logFC). Lines represent used cutoff values to identify the most hyper-methylated DMPs (red) and hypo-methylated DMPs (green). (f) DNA sequences motifs identified to be enriched in DMRs by CisGenome Browser software. The right column contains the top three transcription factor similar to these motifs using JASPAR database. (g) Gene enrichment analysis of gens with DMPs in RPL.
Article Snippet: After performing bisulfite treatment with
Techniques: DNA Methylation Assay, CpG Methylation Assay, Methylation, DNA Methylation ChIP, Software
Journal: EBioMedicine
Article Title: Integrated analysis of DNA methylome and transcriptome identified CREB5 as a novel risk gene contributing to recurrent pregnancy loss
doi: 10.1016/j.ebiom.2018.07.042
Figure Lengend Snippet: Filtration of genes with synergetic alterations in DNA methylation and gene expression. (a) Quadrant plot showing DMRs and expression of corresponding genes. The dashed lines indicate a threshold of P value below 0·05. The blue dots signify hypo-methylated and up-regulated genes in RPL, the brown bots signify hypo-methylated and down-regulated genes in RPL, the purple bots signify hyper-methylated and up-regulated genes in RPL and the red bots signify hyper-methylated and down-regulated genes in RPL. (b) Box plots of relative expression level of four genes selected by a threshold of 5% FDR. Error bars, SEM. * P < 0·05, ** P < 0·01, *** P < 0·001. Bisulfite sequencing of CREB5 (c) and RBM24 (d). Open and closed circles represent unmethylated and methylated CpG sites and each row corresponds to a single clone sequenced. All loci shown in picture were found to be hypo-methylated in RPL according to the analysis of Infinium Human Methylation 850 K BeadChip data. (e) The expression of CREB5 protein in control group and RPL group.
Article Snippet: After performing bisulfite treatment with
Techniques: Filtration, DNA Methylation Assay, Expressing, Methylation, Methylation Sequencing